Fig. 5

MIAT acted as miR-141 sponge in GC cells. a BGC-823 cells were transfected with si-control, si-MIAT-1 or si-MIAT-2 for 24 h, expression of miRNAs was measured. b BGC-823 cells transfected with si-MIAT or control vector lentivirus were injected into the right flank and left flank of nude mice, respectively. The expression of miR-141 in tumor lysates was determined. c Sequence alignment of miR-132 with the putative binding sites within the wild-type regions of MIAT-1. BGC-823 cells were co-transfected with miR-141 mimic and MIAT-1-WT vector or MIAT-1-MUT vector for 48 h, the luciferase activity was measured. d WT and the mutated forms of miR-141 sequence were shown. Detection of MIAT using real-time PCR in the sample pulled down by biotinylated miR-141. e The RIP assay was performed to confirm whether MIAT and miR-141 could directly bind to AGO2 in BGC-823 cells. f BGC-823 cells were transfected with si-control or si-AGO2 for 24 h. Expression of MIAT and miR-141 was measured. **P < 0.01, compared to si-control, pre-NC, Bio-NC or Anti-IgG