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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Long non-coding RNA MIAT promotes gastric cancer growth and metastasis through regulation of miR-141/DDX5 pathway

Fig. 6

DDX5 was a novel target of miR-141 in GC cells. a The 3’-UTR of DDX-5 harbored miR-141 cognate site. b BGC-823 cells were co-transfected with miR-141 mimic and wild-type (WT) or mutant DDX-5 3’-UTR for 48 h, the luciferase activity was determined. c BGC-823 cells were transfected with pre-NC or miR-141 mimic for 48 h, the mRNA level of DDX-5 was determined. d BGC-823 cells were transfected with pre-NC or miR-141 mimic for 48 h, protein level of DDX-5 was determined. e BGC-823 cells were co-transfected with pcDNA-MIAT (MIAT), miR-141 mimic and DDX-5 3’-UTR for 48 h, the luciferase activity was determined. f pcDNA-MIAT (MIAT) with or without miR-141 mimic were transfected into BGC-823 cells for 48 h, protein level of DDX-5 was determined. g miR-141 mimic with or without pcDNA-MIAT (MIAT) were transfected into BGC-823 cells for 48 h, DDX-5 protein level was determined. **P < 0.01, compared to Pre-NC. ##P < 0.01, compared to miR-141 mimic + pcDNA

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