Fig. 6

miR-148b-3p inhibits GC metastasis by decreasing the expression of Dock6. a Transwell assay analyses of the migration and invasion abilities of the indicated GC cells. Scale bars, 50 μm. ^**P < 0.01, ^***P < 0.001. b-f In vivo tail vein metastatic assay. The indicated GC cells were injected into the tail vein of nude mice, followed by noninvasive bioluminescence imaging and H&E staining of lung tissues at 10 weeks after the injection. Scale bars represent 500 μm (low magnification) and 50 μm (high magnification). ^*P < 0.05. g-k In vivo spleen metastatic assay. The indicated GC cells were injected into the spleen of nude mice, followed by noninvasive bioluminescence imaging and H&E staining of liver tissues at 10 weeks after the intrasplenic transplantation. Scale bars represent 500 μm (low magnification) and 50 μm (high magnification). ^*P < 0.05