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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: BRD7 expression and c-Myc activation forms a double-negative feedback loop that controls the cell proliferation and tumor growth of nasopharyngeal carcinoma by targeting oncogenic miR-141

Fig. 3

Restoring miR-141 levels rescued c-Myc knockdown-mediated cell proliferation inhibition and apoptosis promotion in NPC cells. a MTS assays of c-Myc knockdown NPC cells transfected with miR-141 or miR-NC. Ctrl: shCtrl+miR-NC; shc-Myc: shc-Myc + miR-NC. b Colony-forming assay images (lower panel) and quantification of colony number percentages (upper panel). c and d Cell cycle analysis and e Annexin V-PE and 7-AAD double staining analysis of cell apoptosis by flow cytometry. f Expression of the apoptosis marker C-PARP was determined by western blotting. β-actin served as an internal control. a-e The error bars represent the mean ± S.E.M. *P < 0.05, **P < 0.01, ***P < 0.001, N.S=No Significance

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