Fig. 5

c-Myc regulated NPC growth through the miR-141/PTEN/AKT pathway. a qRT-PCR assays confirmed the level of miR-141 restoration in c-Myc knockdown 5-8F and HNE1 cells. U6 served as an internal control. b Western blotting confirmed the expression of differently expressed proteins downstream in the PTEN/AKT signaling, including p27, CCND1, CDK4, Caspase9, and Cleaved-caspase9, in miR-141-restoring 5-8F and HNE1 cells and the controls. β-actin served as an internal control. Ctrl: shCtrl+miR-NC, shc-Myc: shc-Myc + miR-NC. c IHC (DAB staining) for PTEN, pAKT, p27, and CCND1 in the 5-8F xenografts. Three tumors were analyzed per group using IHC staining for the molecules mentioned above. Original magnification, 200×; the scale bar represents 50 μm