5′/ 3′ imbalance strategy to detect ALK fusion genes in circulating tumor RNA from patients with non-small cell lung cancer

Table 1 The primer and probe for detecting the 3′/5′ portion of ALK gene with RT-qPCR

Gene	Exon	Primer(5′ → 3′)	Located on cDNA	Amplicon length
ALK	E20	Forward: CGGCATCATGATTGTGTACC	3159~ 3178	145 bp
		Reverse: CTTGCCAGCAAAGCAGTAG	3285~ 3303
		Probe: FAM-CTGAGCAAGCTCC-MGB	3238~ 3303
	E3	Forward: CAGCCGATATGGTCTGGAG	777~ 795	168 bp
		Reverse: ATCTCCTTAGAACGCTCTGC	925~ 944
		Probe: VIC-TCCCCTCCACTGCAT-MGB	829–843
ACTB	E3	Forward: CAGGCACCAGGGCGTGAT	114~ 131	134 bp
		Reverse: CCATGTCGTCCCAGTTGGT	229~ 247
		Probe: NED-ACGAGGCCCAGAGCA-MGB	167~ 181

ISSN: 1756-9966