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Table 2 Correlation of the expression of ALK fusion gene detected by RT-qPCR and FISH

From: 5′/ 3′ imbalance strategy to detect ALK fusion genes in circulating tumor RNA from patients with non-small cell lung cancer

Cases RT-qPCRa FISH Pathol.
expression value +/− +/− %
021 33.6 + + 18 A
124 439.1 + + 68 AS
065 123 + + 38 A
075 263 + + 53 AS
184 58.9 + + 23 A
046 49 + + 20 A
089 67.9 + + 27 A
102 759.9 + + 90 A
018 132.35 + + 41 A
068 236.5 + + 49 A
135 468.3 + + 73 A
006 523.1 + + 77 A
029 536.2 + + 79 A
173 458.2 + + 70 AS
084 369.1 + + 61 A
003 786.2 + + 92 A
044 865.4 + + 93 A
148 385.2 + + 63 A
039 46.2 + + 20 A
017 56.8 + + 23 A
  1. NOTE: +, positive; −, negative; Pathol., pathology; A, adenocarcinoma; AS, adenosquamous carcinoma; aExpression in 3′ portion of ALK mRNA relative to 5′ portion and expression value = 2-CT = 2-[(CT(ALK E20)- CT(ACTB))-(CT(ALK E3)-CT(ACTB))]