Fig. 2

Proteomics analysis of DFO- or Dp44mT-treated cells vs untreated cells. a Volcano plot showing the results of differentially expressed proteins based on fold change versus t-test probability (Welch difference). Each protein is represented as a dot and is mapped according to its fold change on the ordinate axis (y), with t-test p-value on the abscissa axis (x). The blue and orange dots indicate up-regulated and down-regulated proteins, respectively. Grey dots have identification which did not meet the fold and p-value criteria. Some key molecules that changed significantly as a result of the treatments are indicated. Bubble graphs showing the top 12 down-regulated (panel b) and the top 12 up-regulated (panel c) KEGG pathway terms plotted against the -log₁₀ (p-value) for proteins obtained from whole-cell lysate from untreated MDA-MB-231 cells compared with those from DFO- or Dp44mT-treated cells. The size of bubbles represents the number of proteins enriched for each KEGG pathway term. All pathways had a false discovery rate (FDR) < 0.001