Fig. 4From: Lipid accumulation in human breast cancer cells injured by iron depletorsCell phenotype represents marked dilatation of the rough endoplasmic reticulum (ER). a Cells were treated with DFO or Dp44mT for 48 h, processed for immunofluorescence, and then observed under a fluorescence microscope. The vacuoles were identified to be positive for late endosome/macropinosome markers RAB7 and LAMP1. Stars indicate the localisation of RAB7 and LAMP1 surface proteins in the membranes of vacuoles. TfR was used as a marker for endocytosis. b Western blotting analysis of the expression of the epithelial to mesenchymal transition markers NDRG1, beta-catenin, and vimentin. The protein lysates were obtained from cells treated with DFO or Dp44mT for the times indicated. c Localisation of NDRG1 and RTN4 in vacuolated MDA-MB-231 cells. Cells were treated with DFO or Dp44mT for 48 h, processed for immunofluorescence, and then observed under a confocal microscope. Cells were immunostained with NDRG1 and RTN4 (red) and anti-tubulin (green) antibodies and then analysed by fluorescence microscopy. Blue, DAPI. Scale bars: 100 μmBack to article page