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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Lipid accumulation in human breast cancer cells injured by iron depletors

Fig. 4

Cell phenotype represents marked dilatation of the rough endoplasmic reticulum (ER). a Cells were treated with DFO or Dp44mT for 48 h, processed for immunofluorescence, and then observed under a fluorescence microscope. The vacuoles were identified to be positive for late endosome/macropinosome markers RAB7 and LAMP1. Stars indicate the localisation of RAB7 and LAMP1 surface proteins in the membranes of vacuoles. TfR was used as a marker for endocytosis. b Western blotting analysis of the expression of the epithelial to mesenchymal transition markers NDRG1, beta-catenin, and vimentin. The protein lysates were obtained from cells treated with DFO or Dp44mT for the times indicated. c Localisation of NDRG1 and RTN4 in vacuolated MDA-MB-231 cells. Cells were treated with DFO or Dp44mT for 48 h, processed for immunofluorescence, and then observed under a confocal microscope. Cells were immunostained with NDRG1 and RTN4 (red) and anti-tubulin (green) antibodies and then analysed by fluorescence microscopy. Blue, DAPI. Scale bars: 100 μm

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