Fig. 6

Modifications of morphology and protein expression observed in the cell line MDA-MB-157 during iron depletion. a MDA-MB-157 cells were treated with 100 μM of the iron chelator DFO for 72 h and photographed under an optical microscope. Scale bars: 100 μm. b MDA-MB-157 cells, untreated or treated with 100 μM DFO for 48 h, were labelled with anti-Rab 7 or anti-TfR antibody (red) followed by appropriate secondary antibodies, anti-tubulin (green), and DAPI (blue) and observed under an Olympus Fluo View 1000 fluorescence microscope. Only representative merged images are shown. Scale bars: 100 μm. c Western blot analyses of DFO- or Dp44mT-treated cells showing increased levels of phosphorylated JNK, beta-catenin, vimentin, and NDGR1. Tubulin was used as a loading control