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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Lipid accumulation in human breast cancer cells injured by iron depletors

Fig. 7

Oil Red O staining and label-free CARS imaging of MDA-MB-231 cells after DFO treatment. a Oil Red O staining of MDA-MB-231 cells treated with DFO for 48 h. Images on the left show cells under a microscope at magnifications of 200× and 400×. Cells were stained with Oil Red O and counterstained with haematoxylin to show nuclei (N). The cells adopted a round morphology and accumulated large cytoplasmic vacuoles (V) in which accumulation of lipids (LD) was interspersed. Images on the right show examples of how cell morphology evolves after 72 h of treatment. Cells shrink before dying with a consistent release of aggregates of floating lipid droplets. b CARS composite images of MDA-MB-231 cells treated with 100 μM DFO for 72 h. Panels show the images of lipids in green (2850 cm− 1) and of the vacuoles and medium (3050 cm− 1); CARS spectra are also reported

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