Fig. 3

Contribution of GDF15 secreted by leukemic cells to the adipocyte remodeling. a Adipocytes treated with rhGDF15 (+GDF15) or neutralizing anti-GDF15 antibody (CM + anti-GDF15) or co-cultured with GDF15 knock down THP-1 cells (Ctr + THP-1 GDF15-KD) were stained with Alexa Fluor 493/503-conjugated BODIPY. Adipocytes cultured alone (Ctr) or cultured with the conditioned medium of THP-1 cells (CM) or co-cultured with negative control (Ctr + THP-1 GDF15-NC) were used as the controls respectively. Scale bar represents 100 μm. b The area of each adipocyte in each group was analyzed by using Image-Pro-Plus 5.1. *P < 0.05, **P < 0.01. c Adipocyte number in each group was analyzed by using Image-Pro-Plus 5.1. *P < 0.05, **P < 0.01. d The adipogenic gene (FABP4, PPARγ, C/EBPα) expression was detected by RT-qPCR analysis in each group. *P < 0.05, **P < 0.01, ***P < 0.001. Results shown are from three independent experiments. Values shown are the mean ± SEM