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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: MiR-10a-5p targets TFAP2C to promote gemcitabine resistance in pancreatic ductal adenocarcinoma

Fig. 3

MiR-10a-5p targets TFAP2C to increase gemcitabine resistance. a Luciferase reporter constructs containing downstream wild-type (WT) and mutated (MUT) binding sequences in TFAP2C; b the luciferase activity of the WT and MUT groups suggests that TFAP2C is a direct target of miR-10a-5p; c miR-10a-5p did not change the TFAP2C mRNA levels in the AsPC-1 cell line; d miR-10a-5p expression up-regulation decreased the TFAP2C protein level in the AsPC-1 cell line; e miR-10a-5p expression did not change the TFAP2C mRNA levels in the T3M4 and Su86.86 cell lines; f miR-10a-5p expression up-regulation decreased the TFAP2C protein level in the T3M4 and Su86.86 cell lines; g TFAP2C expression up-regulation in AsPC-1 cells stably overexpressing miR-10a-5p significantly increased gemcitabine chemosensitivity; h TFAP2C expression up-regulation in AsPC-1 cells stably overexpressing mimics control also significantly increased gemcitabine chemosensitivity; i TFAP2C expression up-regulation in AsPC-1 cells stably overexpressing miR-10a-5p significantly increased gemcitabine-induced cell apoptosis; j TFAP2C up-regulation in AsPC-1 cells stably overexpressing mimics control significantly increased gemcitabine-induced cell apoptosis. The data are presented as the means ± SD (Student’s t-test; *, P < 0.05)

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