Fig. 1
Expression of YAP and its association with clinical pathological features of PDAC. (A) Representative western blot analyses showing YAP expression levels in PDAC and NP tissues. (B) Representative IHC images of normal pancreatic tissue with negative (a) and weak (b) YAP staining, and PDAC with negative (c), weak (d), moderate (e) and strong (f) YAP staining are shown (bar, 100 μm). (C) YAP expression was markedly increased in PDAC compared with NP tissues (P < 0.001). (D) Representative IHC images showing YAP expression levels in PDAC with TNM I and TNM IV (bar, 100 μm). (E) Positive association of YAP expression with TNM stage in PDACs (P < 0.05). (F) Representative hematoxylin and eosin (HE) staining and immunostaining for YAP and Ki-67 in normal pancreatic tissues (NP) from wild-type (WT) mouse, early and late mPanIN pancreatic tissues from LSL-KrasG12D/+; Pdx1-Cre (KC) mouse model and PDAC tissues from LSL-KrasG12D/+; Trp53fl/+; Pdx1-Cre (KPC) mouse model (bar, 100 μm)