Fig. 3

Knockdown of YAP inhibits pancreatic cancer cell invasion through inhibiting EMT a Transwell chamber assays of BxPC-3 and Panc-1 cells. The pretreated cells were seeded into a Matrigel-coated invasion chamber for an additional 48 h. b The invasive cells were quantified by counting the number of cells in 10 random fields at 100 × magnification. c & e Western blot assays were performed to evaluate the effect of YAP-shRNA on the protein expression of E-cadherin, N-cadherin, Vimentin and YAP in BxPC-3 and Panc-1 cells. d & f Real-time PCR was performed to evaluate the effect of YAP-shRNA on the mRNA expression of E-cadherin, N-cadherin and Vimentin. All of the data are presented as the mean ± SD of three independent experiments. Column: mean; bar: SD; *P < 0.05; **P < 0.01