Fig. 4

A simultaneous treatment with palbociclib and PI3K/AKT/mTOR inhibitors induces additive effects. MDA-MB-231 (a-c) and HCC38 cells (d-f) were treated with 0.5 μM palbociclib alone or in combination with increasing concentrations of BYL719, BEZ235 or BKM120. After 72 h cell proliferation was assessed by CV assay. The effect of the drug combinations was evaluated using the Bliss interaction model. Data are mean values ±SD of three independent experiments (n = 5)