Fig. 6

In vivo amoeboid angiogenesis. Effects of uPAR silencing and uPAR-integrin uncoupling in Matrigel Plug Assay. Angiogenesis in a Matrigel plug assay in SCID mice by the subcutaneosly addition of Matrigel containing heparin (50 U/ml) with and without VEGF in both mesenchymal and amoeboid conditions. a In the first lane, a representative photograph of individual Matrigel plugs recovered at autopsy for each condition shown. Angiogenesis was evaluated by hemoglobin (Hb) contents shown in the histograms on the right. Consecutive 5 μm histological sections from Matrigel plugs were recovered 5 days after implantation. The second lane shows hematoxylin/eosin staining. b Angiogenesis in a Matrigel plug assay in SCID mice by the subcutaneosly addition of Matrigel containing heparin (50 U/ml) and protease inhibitor mix. Treatments consisted in plug administration of liposome-encapsulated vehicle alone (DOTAP), DOTAP + scramble ODN (sODN), DOTAP + uPAR-aODN (uPARaODN), scramble M25 peptide (Scramble) and M25 peptide (M25). In the first lane, a representative photograph of individual Matrigel plugs recovered at autopsy for each condition shown. Angiogenesis was evaluated by hemoglobin (Hb) contents shown in the histograms on the right. Consecutive 5 μm histological sections from Matrigel plugs were recovered 5 days after implantation. The second and the third lane show hematoxylin/eosin. Images were captured at Å~ 10 magnification. Pictures shown in the figures (a) and (b) are representative of 2 different experiments performed in duplicate on two mice for each experimental condition, each mouse injected in both flanks