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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: SGK1 inhibition-induced autophagy impairs prostate cancer metastasis by reversing EMT

Fig. 5

SGK1 inhibition-induced autophagy activates MET. (a-b) Quantitative RT-PCR analysis of SGK1, Snail, and Fibronectin (FN1) mRNA expression was performed in SGK1-depleted PC3 and LNCaP cells (shSGK1) and in control cells (LV2-Ctrl). (c) Effect of SGK1 inhibition-induced autophagy on EMT regulators. PC3 and LNCaP cells were infected with empty lentivirus control (LV2-Ctrl) or with the lentivirus SGK1-shRNA (LV2-shSGK1) and subsequently incubated for 48 h before pretreatment with 5 mM 3MA or PBS (control). Western blots were probed for levels of LC3, E-cad, N-cad and Vimentin, and GAPDH was used as a loading control. All results are representative of three experiments and are expressed as the mean ± S.D.; *P < 0.05

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