Fig. 2

GDF15 promotes cervical cancer cell proliferation in vitro and tumor formation in vivo. The proliferation and viability of HeLa and SiHa cells incubated with PBS, HSA(10 ng/ml) or rhGDF15(10 ng/ml) were detected by growth curves (a and b, respectively) and the MTT assay (c and d, respectively). The proliferation and viability of HT-3 cells modified with CRISPR and shRNA were detected by growth curves (e and f, respectively) and the MTT assay (g and h, respectively). Tumor growth curves (i and j, respectively) and tumor weights (k and l, respectively) are shown for HeLa-GDF15 and SiHa-GDF15 cells and control cells (HeLa-GFP and SiHa-GFP). Pictures were of nude mouse with tumors from HeLa-GDF15 and SiHa-GDF15 and their control cells (m and n, respectively). Immuno-histochemical staining results for Ki67 are shown in tumor xenografts of GDF15-overexpressing HeLa and SiHa cells (o and p, respectively) and the quantitative analysis are shown (q and r, respectively). The data were shown as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 vs. control using One-Way ANOVA