Fig. 5

GDF15 induced activation of AKT1 and ERK1/2 in human cervical cancer cell lines. GDF15, PI3K, AKT/p-AKT1, and Erk1/2/p-Erk1/2 were determined by western blotting and Ras-GTP was by immunoprecipitation. Western blotting of cell lysates from HeLa-GFP and SiHa-GFP cells which were incubated with 0%, 25%, 50% and 75% conditioned medium for 24 h (a and b) and the quantitative analysis are shown (c and d). Western blotting of cell lysates from HT-3-crispr-GDF-15 and HT-3-shGDF15 and their control cell lines (e and f) and the quantitative analysis are shown (g and h). N: nuclear protein; M: medium protein. The data were shown as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 vs. control using One-Way ANOVA