Fig. 6

GDF15 stimulated tyrosine phosphorylation of ErbB2. The effects of various inhibitors on HeLa (a) and SiHa (b) cell proliferation and viability was detected by MTT assay and quantitative statistics in the 7th day were present below, respectively. Western blotting of cell lysates from HeLa and SiHa cells which were treated with various factors (c) and the quantitative analysis were shown (d and e). Western blotting of cell lysates from HeLa and SiHa which were treated with rhGDF15 or/and shErbB2 (f) and the quantitative analysis were shown (g and h). GDF15 was immunoprecipitated from cell lysates of GDF15 overexpressing cell lines (HeLa-GDF15 and SiHa-GDF15) and the immunoprecipitation product was immunoblotted with an anti-p-ErbB2 antibody (i). The data were shown as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 vs. control using One-Way ANOVA