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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Next-generation sequencing analysis of receptor-type tyrosine kinase genes in surgically resected colon cancer: identification of gain-of-function mutations in the RET proto-oncogene

Fig. 5

Analysis of the biochemical effects exerted by RET-G533C and RET-P1047S mutants. Immunoblot analysis of RET expression and activity in transfected HEK293 cells (a). Lysates from transfected HEK293 cells were analysed by immunoblot with anti-phosphoY1062 or anti-RET antibody. The activation status of the MAPK pathway was assessed with anti phospho-Y202T204 ERK1/2 antibody. Anti-ERK1/2 antibody was used for normalization. b. Immunoblot analysis of RET dimers in transfected HEK293 cells. Samples were prepared under non-reducing (NR) or reducing (R) conditions to detect RET dimer formation and loaded onto SDS-PAGE in non-reducing conditions. Half the total amount of protein lysate was loaded in the case of RET634

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