Fig. 2

SP1 binds to the promoter region of SPRY4-IT1 and activates its transcription. a SP1 binding site prediction in the SPRY4-IT1 promoter region using JASPAR. b RT-qPCR analysis of SPRY4-IT1 and SP1 expression in HuCCT1 and RBE cells after SP1 silencing. c RT-qPCR analysis of SPRY4-IT1 expression in HuCCT1 and RBE cells after SP1 overexpression. d ChIP-qPCR analysis of SP1 occupancy in the SPRY4-IT1 promoter in HuCCT1 and RBE cells. ANRIL was used as a positive control and IgG as a negative control. e Construction of the luciferase reporter vector. f Dual luciferase reporter assays were used to determine the SP1 binding site on the SPRY4-IT1 promoter region. *P < 0.05, **P < 0.01