Skip to main content
Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: SP1-induced upregulation of lncRNA SPRY4-IT1 exerts oncogenic properties by scaffolding EZH2/LSD1/DNMT1 and sponging miR-101-3p in cholangiocarcinoma

Fig. 3

SPRY4-IT1 knockdown inhibits CCA cell growth in vitro and in vivo. a Relative expression of SPRY4-IT1 in HuCCT1 and RBE cells measured by RT-qPCR. b Proliferation curves were determined in HuCCT1 and RBE cells after transfected with si-SPRY4-IT1–1, si-SPRY4-IT1–2 or si-NC by CCK-8 assays. c The location and relative expression of Ki67 were detected in HuCCT1 and RBE cells after transfected with si-SPRY4-IT1–1, si-SPRY4-IT1–2 or si-NC by Ki67 immunofluorescent staining assays. Ki67 positive cells were labeled with green fluorescence; nuclear fractions were labeled with DAPI (blue). d Colony-forming abilities were measured in HuCCT1 and RBE cells after transfected with si-SPRY4-IT1–1, si-SPRY4-IT1–2 or si-NC by clonogenic assays. e Cell apoptosis was detected in HuCCT1 and RBE cells after transfected with si-SPRY4-IT1–1, si-SPRY4-IT1–2 or si-NC by flow cytometry. f Cell apoptosis was detected in HuCCT1 and RBE cells after transfected with si-SPRY4-IT1–1, si-SPRY4-IT1–2 or si-NC by TUNEL staining assays. Apoptotic cells were labeled with TUNEL (green); nuclear fractions were labeled with DAPI (blue). g Relative expression of caspase-3 and caspase-9 were detected in HuCCT1 and RBE cells after transfected with si-SPRY4-IT1–1, si-SPRY4-IT1–2 or si-NC by caspase-3/9 activity assays. h 18 days after injection of HuCCT1 cells transfected with shSPRY4-IT1 or shCtrl, tumors were removed from nude mice. i Tumor volumes were measured every 3 days after injection. j Tumor weights were measured after the tumors were harvested. k The tumor sections were under IHC staining using antibodies against Ki67. l RT-qPCR was conducted to determine the average expression of SPRY4-IT1 in xenograft tumors. *P < 0.05, **P < 0.01

Back to article page