Fig. 4

SPRY4-IT1 promotes cell metastatic properties by reversing EMT. a The migration capacities were detected in HuCCT1 and RBE cells transfected with si-SPRY4-IT1–1, si-SPRY4-IT1–2 or si-NC by wound healing assays. b-c The migration and invasive capacities were detected in HuCCT1 and RBE cells transfected with si-SPRY4-IT1–1, si-SPRY4-IT1–2 or si-NC by transwell assays. d E-cadherin, Snail, vimentin, Bcl-2 and Bax protein levels were detected in HuCCT1 and RBE cells transfected with si-SPRY4-IT1–1, si-SPRY4-IT1–2 or si-NC by western blotting. *P < 0.05, **P < 0.01