Fig. 6

SPRY4-IT1 promotes CCA cell proliferation and invasion in part by regulating KLF2 and LATS2 expression. a Western blot assays were used to determine the expression of KLF2 and LATS2 in HuCCT1 and RBE cells. b-e CCK-8, colony formation, flow cytometric and transwell assays were used to determine the viability, colony forming ability, apoptosis and invasion of HuCCT1 and RBE cells. f Western blot assays were used to determine the expression of KLF2 and LATS2 in HuCCT1 and RBE cells. g-j CCK-8, colony formation, flow cytometric and transwell assays were used to determine the viability, colony forming ability, apoptosis and invasion of HuCCT1 and RBE cells. k Summary of the regulation and mechanism of SPRY4-IT1 in CCA cells. *P < 0.05, **P < 0.01