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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: SPIN1, negatively regulated by miR-148/152, enhances Adriamycin resistance via upregulating drug metabolizing enzymes and transporter in breast cancer

Fig. 3

The expression of ABCB4, CYP2C8, UGT2B4 and UGT2B17 was upregulated by SPIN1 in breast cancer cells. a Candidate SPIN1 targets from microarray were presented as a heat map. b-c The mRNA expression levels of ABCB4, CYP2C8, UGT2B4, and UGT2B17 were decreased after SPIN1 siRNA transfection. Accordingly, overexpression of SPIN1 led to upregulation of ABCB4, CYP2C8, UGT2B4, and UGT2B17 mRNA expression levels. d-e Western blot analysis confirmed that overexpression of SPIN1 upregulated ABCB4, CYP2C8, UGT2B4, and UGT2B17 protein levels, however, SPIN1 downregulation decreased the expression levels of these proteins. f-i Data from PPISURV (ABCB4 and UGT2B4) and GOBO (CYP2C8 and UGT2B17) revealed that patients with high expression of ABCB4, CYP2C8, UGT2B4 or UGT2B17 had poorer survival than those with low expression

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