Fig. 4

CX-5461 altered hTERT alternative splicing in GBM cells. a The expression of hTERT transcripts were investigated by RT-PCR analysis. CX-5461 (1 μM, 48 h) markedly altered its splicing patterns, leading to a sharp decrease of hTERT+β transcript in T98G and U251 cells. While TMPyP4 and BRACO-19, two other G-quadruplex stabilizers, did not significantly alter its splicing patterns. b The histogram showed transcripts expression levels relative to control. c CX-5461 could lead to a decrease of hTERT+β transcript and to an increase of hTERT-β in a dose-dependent manner in T98G and U251 cells. d The histogram showed transcripts expression levels relative to control. e CX-5461 could lead to a significant decrease of hTERT-FL in a dose-dependent manner and had no effect on hTERT-All. f The histogram showed transcripts expression levels relative to control. *P < 0.05, **P < 0.01 vs. control group