Fig. 5

CX-5461 weakly decreased hTERT protein expression, but effectively inhibited telomerase activity in GBM cells. a The hTERT protein was detected both in T98G and U251 cells by immunoblotting after treatment with CX-5461 for 48 h, and β-actin was used as loading control. b The histogram showed hTERT protein expression levels relative to control. P>0.05 vs. control group. c After treatment with CX-5461 for 48 h, the telomerase activity of GBM cells was detected by TRAP assay. d The histogram showed a significant dose-dependent telomerase activity inhibition of CX-5461 in GBM cells. *P < 0.05, **P < 0.01