Fig. 7

Short-term apoptosis was evoked by CX-5461-induced DNA damage response. a, b CX-5461 could induce apoptosis in a dose-dependent manner in T98G and U251 cells. *P < 0.05 vs. control group. c After treatment for 48 h, CX-5461 induced a strong increase of γ-H2AX, 53BP1, and p-ATM, which are hallmarks of DNA double-strand break response. d The expression of Bcl-2 was significantly decreased, meanwhile the activities of cleaved caspase-3 and Bax were markedly elevated compared with the control group