Fig. 4

NZ001, a novel dual inhibitor of MET and VEGFR2, inhibited capillary tube formation of HUVECs and HGF-induced migration and invasion of HCC cells. a The molecular structure of NZ001. b The inhibitory effects of NZ001 on the kinase activities of MET and VEGFR2 and IC50 values determined by KinaseProfiler IC50 Express (Millipore). c NZ001(1umol/l) significantly inhibited VEGF(50 ng/ml)-driven capillary-like structures of HUVECs. d NZ001(1umol/l) significantly inhibited HGF(10 ng/ml)-stimulated migration in a wound-healing assay. e NZ001(1umol/l) significantly inhibited HGF(10 ng/ml)-induced cell invasion in a Transwell assay

Data are shown as the mean ± SD. Significant differences were determined using one-way ANOVA. *: P < 0.05; **: P < 0.01; NS: No Significance.