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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Patient-derived multicellular tumor spheroids towards optimized treatment for patients with hepatocellular carcinoma

Fig. 3

MCTS formation and drug sensitivity of primary HCC cells. a Schematic of MCTS model representing the in vivo microenvironment. b Morphology of tumor spheroids and MCTS of AMC-H1 and AMC-H2 HCC cells. c Drug sensitivities in MCTS of AMC-H1 and AMC-H2 HCC cells grown with hepatic stellate cells (HSC; LX2), fibroblasts (WI38), and endothelial cells (HUVECs). Cells were cultured in 96-well ULA plates for 3 day and then treated with 5-FU, cisplatin, and sorafenib at the indicated concentrations for 7 day. Then, MCTS were stained with EthD-1 (upper panel), and the staining intensity was analyzed (lower panel). d EthD-1 intensity of tumor spheroids and MCTS composed with AMC-H1 or AMC-H2 after treatment with the indicated drugs. All images were obtained and analyzed using the HCS system. Data are shown as the mean ± SD of triplicate experiments. Scale bar = 200 μm. *p < 0.05, **p < 0.005

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