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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: The phospholipase DDHD1 as a new target in colorectal cancer therapy

Fig. 1

Effects of DDHD1 silencing on tumor and non-tumor cell viability and apoptosis. a, c Cell viability was measured by MTT assay on SW480, HCT116, HS5 and HUVEC cell lines after 48 or 72 h of transfection with scrambled siRNA or with DDHD1 siRNA. The values were plotted as % of growth vs Ctrl (non transfected cells). Each point represents the mean ± SD of three independent experiments. b, d Cell death was detected by Annexin V staining after 72 h of transfection with scrambled siRNA or with DDHD1 siRNA. Histogram reported % of Annexin V positive cells in transfected samples compared to untreated (Ctrl). Asterisks indicate statistically significant values in comparison to cells transfected with scrambled siRNA (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001)

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