Fig. 4

Chromosome aberrations induced by A35. K562 cells were treated with 2 μM A35 for 24 h, 100 nM UCN-01 was added, and culturing continued for 6 h to abrogate M-phase arrest prior to sample collection. Colcemid (100 ng/mL) was then added to the cell cultures 2 h prior to harvesting. a Representative images of chromosome spreads. Arrows, chromosome breaks. b Quantitation and comparison of chromosome aberrations. p < 0.01 vs Control or UNC-01 alone, # p = 0.1 vs Control