Fig. 3

Bortezomib inhibits activation of TRAF6 and NF-κB activity. a After the cells were exposed to IR, they were collected and lysed. The expression of phosphorylated ATM, p65 and γH2AX were examined by Western blot analysis. b SAS cells were treated with IR, lysed at the times indicated (min), and immunoprecipitated with TRAF6 antibody. IP extracts were analyzed for ubiquitin (Ub) or TRAF6 by immunoblotting. c Bortezomib suppressed TRAF6-mediated polyubiquitination by IR. Cells were treated with IR and bortezomib alone or in combination. Cells were treated with 6 Gy of IR or 25 nM of bortezomib for 40 min. d Bortezomib inhibited the activation of NF-κB induced by IR. Cells were treated with IR and bortezomib alone or in combination. Cells were treated with 6 Gy of IR or 25 nM of bortezomib for 1 h