Fig. 4

HSP27 affected the expression and location of AR. Verification of the siRNA specific for HSP27 at three sites (4894-2, 4893-1, 4892-1) was measured by qPCR (a) and western blot (b). The proliferation ability of HSP27 knock-down cells treated with or without DHT was measured by CCK8 (c) and clonogenic (d) assays. The effect of HSP27 knock-down on AR expression in MDA-MB-453 and MCF7 cells was analyzed by western blot (e) and qPCR (f). Western blot analyzed the level of AR expression in the cytoplasm and nucleus after cells transfected with siRNA specific for HSP27 in MDA-MB-453 (g) and MCF7 (h) cells. *P < 0.05