Fig. 5

Rescue assays validated that TGFBR2 mediated the oncogenic functions of miR-93. a. The expression of TGFBR2 was shown after treatment of miR-93 mimics with or without siRNA for TGFBR2, as detected by RT-qPCR and Western blot. b. Annexin V-FITC apoptotic assays for treated cells. Additional TGFBR2 silencing decreased the cell ratio of early apoptosis in PC3 cells. c. Colony formation assay showed TGFBR2 siRNA resulted in increased capability of colony formation in two PCa cell lines with miR-93 inhibitor. d. Transwell assay showed TGFBR2 siRNA resulted in increased capability of cell migration in two PCa cell lines with miR-93 inhibitor. *, P < 0.01; **, P < 0.01