Fig. 3
From: NHERF1 and tumor microenvironment: a new scene in invasive breast carcinoma
Immunofluorescence analysis of NHERF1 and TME biomarkers expressions in invasive breast cancer. a A representative tissue sample stained with NHERF1 and VEGF, VEGFR1, HIF-1α and TWIST1 antibodies and detected with Alexa Fluor 568 (red) and Alexa Fluor 488 (green) secondary antibodies and nucleus in blue (DAPI stained), respectively, prior to fluorescence microscopy analysis (200× magnification). Arrowheads indicate co-localization sites of NHERF1 with the others markers. b A representative tissue sample stained with NHERF1 and CD34 antibodies and detected with Alexa Fluor 568 (red) and Alexa Fluor 488 (green) secondary antibodies and nucleus in blue (DAPI stained), respectively, prior to fluorescence microscopy analysis. The square includes microvessel formation with a double staining NHERF1-CD34