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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: miR-221 stimulates breast cancer cells and cancer-associated fibroblasts (CAFs) through selective interference with the A20/c-Rel/CTGF signaling

Fig. 1

miR-221 regulates A20 expression in CAFs and breast cancer cells. a Expression of miR-221 in fibroblasts, CAFs, MCF10A, MDA-MB 231 and SkBr3 cells. Raw Ct data were normalized to the housekeeping RNU6 levels and expressed as ΔCt values using the comparative cross threshold (Ct) method. b miR-221 expression in CAFs, MDA-MB 231 and SkBr3 breast cancer cells after transfection for 48 h with 25 nM miR-Ctrl and 25 nM miR-221, alone or in combination with 100 nM LNA-i-miR-221 (LNA-i). c Schematic alignment between the miR-221 sequence and the 3’-UTR mRNA region of A20. d A20 mRNA expression in CAFs, MDA-MB 231 and SkBr3 cells transfected for 48 h with 25 nM miR-Ctrl and 25 nM miR-221, alone or in combination with 100 nM LNA-i-miR-221 (LNA-i). A20 protein expression in CAFs (e), MDA-MB 231 (f) and SkBr3 (g) cells transfected for 48 h with 25 nM miR-Ctrl and 25 nM miR-221, alone or in in combination with 100 nM LNA-i-miR-221 (LNA-i); β-actin serves as a loading control. Below panels show densitometric analysis of the blots normalized to the loading controls. Each column represents the mean ± SD of three independent experiments performed in triplicate. The data are shown as fold induction respect to cells transfected with miR-Ctrl. (*) indicates p < 0.05 and (**) p < 0.01

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