Fig. 3

PDK1 mediates Chibby-regulated aerobic glycolysis. a Western blotting analysis of metabolic enzymes in Chibby-overexpressed SUNE-1 and CNE2 cells. b mRNA levels of PDK1 were determined by quantitative polymerase chain reaction in Chibby-overexpressed SUNE-1 and CNE2 cells. c protein levels of PDK1 were determined by western blot in Chibby-knockdown CNE1 and HK-1 cells. d Cellular glucose uptake and lactate release levels were measured in Chibby-overexpressed SUNE-1 and CNE2 cells after PDK1 overexpression. e Cellular glucose uptake and lactate release levels were measured in Chibby-knockdown CNE1 and HK-1 cells following knockdown of PDK1. Data were expressed as the mean ± SD, n = 3 for each group. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant