Fig. 4

PDK1 is positively regulated by Wnt/β-catenin signaling via Lin28/Let7. a Western blotting analysis of Lin28 and PDK1 in β-catenin-overexpressed CNE1 and HK-1 cells. b mRNA levels of Lin28, let-7 g, and PDK1 were determined by quantitative polymerase chain reaction in β-catenin-overexpressed CNE1 and HK-1 cells. c Western blotting analysis of Lin28 and PDK1 in β-catenin-overexpressed CNE1 and HK-1 cells after Lin28 knockdown or Let-7 g overexpression. d Western blotting analysis of Lin28 and PDK1 in β-catenin-knockdown SUNE-1 and CNE2 cells after Lin28 overexpression or Let-7 g antagomir treatment. Data were expressed as the mean ± SD, n = 3 for each group. *, p < 0.05; **, p < 0.01; ns, not significant