Fig. 2

LncMAPK6 is overexpressed in liver tumor and TICs. (a) MAPK6 locus was shown. There is a divergent lncRNA (ENST00000561318, termed as lncMAPK6 in this research) in near from MAPK6 locus according to UCSC Genome Browser. (b) LncMAPK6 expression levels were detected using Northern blot. 18S rRNA was a loading control. (c) 19 peri-tumor, 7 eHCC and 12 aHCC were used for In situ hybridization (ISH). Representative photos and indicated ratios were shown. (d) LncMAPK6 expression levels in CD133⁺ liver TICs and CD133⁻ non-TICs were detected by realtime PCR, and non-TIC expression was used for data normalization. (e) Spheres and non-spheres were selected, and lncMAPK6 expression was examined with realtime PCR. (f, g) FISH assay confirmed the high expression of lncMAPK6 in liver TICs (f) and oncospheres (g). Zic2 and CD133 were markers of liver TICs. Scale bars, C, 50 μm; F, 20 μm