Fig. 3
miR-600 suppressed EMT related proteins in CRC cells and inhibited CRC growth in vivo. a qRT-PCR showed miR-600 suppressed EMT in CRC cells. Overexpression of miR-600 suppressed N-cadherin, vimentin and Fibronectin expressions, and accelerated E-cadherin expression in SW620 and LOVO cells. Inhibition of miR-600 accelerated N-cadherin, vimentin and Fibronectin expressions, and suppressed E-cadherin expression. b Western blot analysis of the effect of miR-600 on E-cadherin, N-cadherin, vimentin and Fibronectin expressions. c SW620 cells (transfected with lenti-miR-600 or lenti-vector) were subcutaneously injected into each BALB/c nude mice at a concentration of 1 × 106 cells/100 μl (n = 6). Seven days after inoculation, tumor volume was measured. Nude mice were sacrificed after 25 days, then tumor tissues were weighed. miR-600 expression was increased in lenti-miR-600 group, and overexpression of miR-600 significantly suppressed tumor volume and weight. d SW620 cells (transfected with lenti-miR-600 or lenti-vector) were injected into the spleen subcapsular of each BALB/c nude mice at a concentration of 1 × 106 cells/100 μl (n = 6) to establish nude mice liver metastasis model of CRC. Nude mice were sacrificed after 5 weeks, and liver tissues were obtained to count tumor nodules. Overexpression of miR-600 significantly decreased the number of metastatic nodules after spleen injection