Fig. 6

TUG1 regulated miR-600 expression and influence the effect of miR-600 on the migration of CRC cells. Bioinformatics software (DIANA) predicted binding sites between TUG1 and miR-600. a Binding sites between TUG1 and miR-600. Luciferase reporter gene vector containing TUG1 WT or TUG1 Mutant were co-transfected with miR-600 mimic or pre-NC into SW620 cells. Dual-luciferase reporter gene assay showed that miR-600 mimic significantly decreased luciferase activity of TUG1 WT, and there was no significant change in the activity of TUG1 Mutant, indicating miR-600 can bind with TUG1. b qRT-PCR showed that si-TUG1 significantly decreased TUG1 level and increased miR-600 level in SW620 and LOVO cells. c pcDNA-TUG1 significantly increased TUG1 level and decreased miR-600 level in HCT116 cells. d Ago2 antibody was added into SW620 cell lysate for RIP. qRT-PCR showed that TUG1 and miR-600 enrichment was significantly increased in Ago2 than IgG. e TUG1 level was negatively correlated with miR-600 level in CRC tissue. f-g SW620 cells were transfected with si-NC, si-TUG1, si-TUG1 + NC, si-TUG1 + miR-600 inhibitor. Transwell assay showed that miR-600 inhibitor reversed the inhibition effect of si-TUG1 on the migration and invasion of CRC cells. Western blot analysis showed that miR-600 inhibitor reversed the effect of TUG1 on EMT related protein expression