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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Physical interaction of STAT1 isoforms with TGF-β receptors leads to functional crosstalk between two signaling pathways in epithelial ovarian cancer

Fig. 2

TGF-β1 regulates the phosphorylation of STAT1 in ovarian surface epithelial cells. Dose-dependent (a, b) and time-course (c, d) studies of the effect of TGF-β1 on STAT1 phosphorylation (pSTAT1) are shown. a Immunoblotting after cells treated with TGF-β1 (0, 0.1, 1, and 10 ng/ml) for 24 h. b Densitometry analysis of the gels in (a). c Immunoblotting after cells treated with TGF-β1 (10 ng/ml) for 30, 60, and 180 min. d Densitometric analysis of the gels in (c). The phosphorylation of Smad2 (pSamd2) is increased after TGF-β1 treatment, indicating the responsiveness of cells to TGF-β1. Data are represented as the mean ± SEM of the ratio of pSTAT1/total STAT1 and total STAT1/β-actin. n = 3 independent experiments; *, P < 0.05 compared to untreated cells

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