Fig. 3

Inhibition of TGF-β receptor kinase blocks the effect of TGF-β on STAT1 phosphorylation in ovarian surface epithelial cells. Cells were pretreated with 10 μM TβRI inhibitor SB-431542 for 30 min, followed by 10 ng/ml of TGF-β1 treatment for 24 h. a Immunoblotting after cells treated with SB-431542 and/or TGF-β1. b Densitometric analysis of the gels in (a). The responsiveness of cells to TGF-β1 was confirmed by the detection of pSmad2. Data are represented as the mean ± SEM of the ratio of pSTAT1 over total STAT1 or total STAT1 over β-actin. NC, non-treated control; SB, SB-431542; n = 3 independent experiments; *, P < 0.05 compared to NC