Fig. 4
STAT1 interacts with the TGF-β1 receptor. HEK-293 T cells were transiently co-transfected with STAT1α-myc or STAT1β-myc and ALK1-HA, ALK5-HA or TβRII-HA plasmids and incubated for 48 h. a Interaction of STAT1α with receptors detected by immunoprecipitation (IP) with anti-myc antibody for STAT1α, followed by immunoblotting (IB) with anti-HA antibody for receptors (upper panel), or IP with anti-HA antibody for receptors, followed by IB with anti-myc antibody for STAT1α (bottom panel). b IB using antibodies specific to β-actin, myc for STAT1α, and HA for receptors. c Interaction of STAT1β with receptors detected by IP with anti-myc antibody for STAT1β, followed by IB with anti-HA antibody for receptors (upper panel), or vice versa (bottom panel). d IB using antibodies specific to β-actin, myc for STAT1β, and HA for receptors. pcDNA4 and pCMV5 are two empty vectors used as negative controls. Each experiment is repeated at least once. Representative images are shown. STAT1α-myc, 93 kDa; STAT1β-myc, 86 kDa; ALK1-HA, 58–69 kDa; ALK5-HA, 53 kDa; TβRII-HA, 71–80 kDa