Fig. 6
STAT1 affects Smad2 phosphorylation. a Immunoblotting after SK-OV-3 cells transiently transfected with pcDNA4, STAT1α or STAT1β plasmid in the absence or presence of TGF-β1 (10 ng/ml) for 24 h. b Densitometric analysis of the gels in (a). c Immunoblotting of STAT1 and Smad2 in STAT1-knockdown SK-OV-3 cells in the absence or presence of TGF-β1 (10 ng/ml) for 24 h. d Densitometric analysis of the gels in (c). Data are represented as the mean ± SEM of the ratio of pSmad2/total Smad2, total Smad2/β-actin, and total STAT1/β-actin. Different superscript denotes statistically significant difference (P < 0.05; n = 3 independent experiments)