Fig. 4

a EpCAM-positive and CD133-positive cells were evaluated by flow cytometry in the “benign tissues” adjacent to tumor mass as well as in non-tumor tissue. Western blot analysis of EpCAM and β-catenin protein levels in liver tissues from 4 experimental groups at month 10. b EpCAM-positive and CD133-positive cells were evaluated by flow cytometry in FL83B and Hepal-6 cells treated by FFA for 48 h. Time-course study of FGFR4 mRNA and protein levels by qPCR and Western blot in Hepal-6 cells challenged by FFA 0–72 h. Analysis for qPCR and Western blot was presented as the fold ratio over GAPDH control. UT: untreated; CD: control diet; HFD: high fat diet; DEN: N-nitrosodiethylamine; h: hour. *, P < 0.05 vs UT + CD