Fig. 4

FAK is essential for SNCG-enhanced tumor cell migration and invasion. a-b, HCT116 cells were transfected with control siRNA and FAK-specific siRNA-2, -3 for 48 h. Then cells were treated with or without GST-SNCG (1 μmol/L) for migration (a) or invasion (b). c-d, HCT116 cells were treated with PBS, 50 μmol/L FAK inhibitor 14 for 30 min and then treated with or without GST-SNCG (1 μmol/L) for migration (c) or invasion (d). Graphed data represent the mean ± SE from at least six 200-power field for each condition, two-sample t-test.e-f, HCT116 cells were transfected with control siRNA (lane 1-2), and FAK-specific siRNA-2 (lane 3-4) and -3 (lane 5-6) for 72 h. cells were treated with or without GST-SNCG (1 μmol/L) for 30 min and cell lysates were analyzed for activated and total β1 integrin (e) or activated and total FAK (f)